Response against Adenoviruses the Brain in the Presence of an Immune Sustain Inducible Transgene Expression in Regulatable Gutless Adenovirus Vectors
نویسندگان
چکیده
2006, 80(1):27. DOI: 10.1128/JVI.80.1.27-37.2006. J. Virol. Castro Donna Palmer, Philip Ng, Pedro R. Lowenstein and Maria G. Kurt Kroeger, Hasmik Agadjanian, Lali Medina-Kauwe, F. Curtin, Gwendalyn D. King, Gabriela Jaita, Chunyan Liu, Marianela Candolfi, Jeffrey M. Zirger, Carlos Barcia, James Weidong Xiong, Shyam Goverdhana, Sandra A. Sciascia, Response against Adenoviruses the Brain in the Presence of an Immune Sustain Inducible Transgene Expression in Regulatable Gutless Adenovirus Vectors
منابع مشابه
Regulatable gutless adenovirus vectors sustain inducible transgene expression in the brain in the presence of an immune response against adenoviruses.
In view of recent serious adverse events and advances in gene therapy technologies, the use of regulatable expression systems is becoming recognized as indispensable adjuncts to successful clinical gene therapy. In the present work we optimized high-capacity adenoviral (HC-Ad) vectors encoding the novel tetracycline-dependent (TetOn)-regulatory elements for efficient and regulatable gene expres...
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Adenoviruses are used extensively to deliver genes into mammalian cells, particularly where there is a requirement for high-level expression of transgene products in cultured cells, or for use as recombinant viral vaccines or in gene therapy. In spite of their usefulness, the construction of adenoviral vectors (AdV) is a cumbersome and lengthy process that is not readily amenable to the generat...
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Glioblastoma multiforme (GBM) is the most common and most aggressive primary brain tumor in humans. Systemic immunity against gene therapy vectors has been shown to hamper therapeutic efficacy; however, helper-dependent high-capacity adenovirus (HC-Ad) vectors elicit sustained transgene expression, even in the presence of systemic anti-adenoviral immunity. We engineered HC-Ads encoding the cond...
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Cellular immune mechanisms that regulate viral gene expression within infected brain cells remain poorly understood. Previous work has shown that systemic immunization against adenovirus after vector delivery to the brain results in complete loss of brain cells infected by adenoviral vectors. Although T cells play an important role in this process, we demonstrate herein that B cells also signif...
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Knowing that adenoviral vectors could initiate innate immunity, the ability of E1-deleted recombinant adenovirus (Ad-E1Δ) in induction of B7.1 and IL-2 molecules was studied. Methods: The expression of green fluorescent protein in C1498 cells following transfection of these cells with adenovirus green fluorescent protein vector confirmed the ability of adenovirus vectors in infecting the cells ...
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